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KMID : 0380020040190050358
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 5 p.358 ~ p.362
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Characteristics of Cell Growth and Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Synthesis by Alcaligenes Latus and Comamonas acidovorans
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Song Jae-Yong
Kim Beom-Soo
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Abstract
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Characteristics of cell growth and poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] synthesis was investigated through flask and batch cultures of Alcaligenes latus and Comamonas acidovorans. The specific growth rate of C. acidovorans increased with yeast extract concentration and decreased with 1,4-butanediol concentration. Optimum glucose concentration for growth of C. acidovorans was 20 g/L. In one-step flask cultures of C. acidovorans, final dry cell weight and PHA content decreased with the ratio of 1,4-butanediol to glucose, while the 4HB fraction in copolymers gradually increased to 100 with an initial 1,4-butanediol concentration of 20 g/L as single carbon source. The specific growth rate of A. latus decreased with v-butyrolactone concentration and optimum sucrose concentration for growth was 10 g/L. In batch cultures of A. latus, 4HB fraction increased with initial v-butyrolactone concentration. P(3HB-co-4HB) with 19 4HB was obtained when the initial ratio of v-butyloractone (g/L) to sucrose (g/L) was 10 : 10.
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KEYWORD
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Poly(3-hydroxybutyrate-co-4-hydroxybutyrate), Alcaligenes Latus, Comamonas acidovorans
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